On the Sampling of Serial Sectioning Technique
نویسندگان
چکیده
Serial sectioning technique provides plenty of quantitative geometric information of the microstructure analyzed, including those unavailable from stereology with oneand two-dimensional probes. This may be why it used to be and is being continuously served as one of the most common and invaluable methods to study the size and the size distribution, the topology and the distribution of topology parameters, and even the shape of three-dimensional space filling grains or cells. On the other hand, requiring tedious lab work, the method is also very time and energy consuming, most often only less than one hundred grains per sample were sampled and measured in almost all reported practice. Thus, a question is often asked: for typical microstructures in engineering materials, are so many grains or cells sampled adequate to obtain reliable results from this technique? To answer this question, experimental data of 1292 contiguous austenite grains in a low-carbon steel specimen obtained from the serial sectioning analysis are presented in this paper, which demonstrates the effect of sampling on the measurement of various parameters of grain size distribution and of the grain topology distribution. The result provides one of rules of thumb for grain stereology of similar microstructures.
منابع مشابه
Topics: 3D CLSM and Light Microscopy, 3D FIB/SEM or Serial Sectioning time resolved microscopy, 3D Image Analysis and simulation 3D TEM Tomography and Serial Sectioning, 3D X-ray Microscopy and Tomography, Ultra-Resolution Tomography Scientific committee and chairpersons:
متن کامل
Application of a Novel Automated Serial Sectioning Technique in the 3D Analysis of Paper Structures
متن کامل
Pattern of Residual Stress in Rail by FEM Analysis and Strain Gage Sectioning Technique
Final step of rail manufacturing is cold rolling straightening process and it hassignificant effects on mechanical properties, straightness, flatness and development of residualstresses. Measurement of residual stresses after straightening process is obligated by railmanufacturing standards. In the present investigation, an attempt has been made to evaluateresidual stresses after straightening ...
متن کاملOn-the-Fly Performance Evaluation of Large-Scale Fiber Track- ing
Large-scale fiber tracking in the images serial-sectioned from material samples is a critical step to analyze physical properties of continuous fiber reinforced composite materials. In serial-section imaging, increasing the sampling sparsity, i.e., the inter-slice intervals, can lead to significant speedups in data collection. However, increasing the sampling sparsity leads to difficulties in t...
متن کاملThe interpretation of granitic textures serial thin sectioning, image analysis three-dimensional reconstruction
Textural development of the felsic phases in two granodioritic rocks from the zoned Linga superunit of the Peruvian Coastal Batholith has been characterized using serial thin sectioning, image analysis and threedimensional reconstruction. The study has shown how each mineral phase contributed to the texture during the formation and development of a contiguous crystal framework and during subseq...
متن کامل